Karnataka

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Project No. : IEC/KR-04/2009-10

Year. : 2009-10

Project Title: Development of a comprehensive electronic database of Indian Medicinal Plants and hosting it on NMPB/website/portal

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Principal Investigator & Organization : Dr. D.K. Ved, FRLHT-IAIM Bangalore, Foundation for Revitalisation of Local Health Traditions (FRLHT)

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Summary of Project / Major Outcome /Achievements : A comprehensive electronic database of Indian medicinal plants has been developed by Foundation for Revitalization of Local Health Traditions (FRLHT) with financial support from National Medicinal Plants Board (NMPB) and it has been hosted on a website which can be accessed at www.medicinalplants.in and linked to NMPB website www.nmpb.nic.in

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Project No. : KR-04/2009-10

Year. : 2009-10

Project Title: Bioactivity guided fractionation of Mangifera indica and Moringa oleifera for developing of standardized herbal extracts for management of cholesterol

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Principal Investigator & Organization : Dr. Amit Agarwal, Natural Remedies Pvt. Ltd., Plot No. 5B, Veerasandra Indl. Area, Electronic City (Post), Bangalore - 560 100

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Summary of Project / Major Outcome /Achievements : Methanol and successive water extracts of Mangifera indica and Moringa oleifera were subjected to screening in various standardized in vitro bioassays, like Bile acid binding, Lipase, Cholesterol esterase, LDL oxidation assays, relevant to cholesterol blocking, to find responding assay for bioactivity guided fractionation. Methanol leaf extract of Mangifera was further fractionated using the guidance of cholesterol esterase inhibition assay. 3β- taraxerol was isolated from an enriched fraction showing an IC50 value of 0.86 μg/ml. 3β- taraxerol was found to be in present in the range of 0.4 - 0.9% w/w in different varieties of mango leaves. The methanolic extract showed cholesterol lowering activity at the dose of 90 mg/kg body weight in high cholesterol diet rat model and also found to be safe up to the dose of 5000 mg/kg body weight. Leaf and fruit/pod methanol extracts of Moringa oleifera and their combinations were tested in in vitro lipase inhibition assay to determine the best combination using fractional inhibitory concentration (FIC). M. oleifera leaf and fruit extracts blended in 1:1 proportion was found to be the best combination in in-vitro lipase inhibition assay.

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Project No. : KR-21/2005-06

Year. : 2005-06

Project Title: Enhancing the productivity of imp. medicinal & aromatic crops grown in mango, sapota & cashew based agroforestry system of farmers through growth promoting micro-organism

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Principal Investigator & Organization : Prof. S.T. Naik, Department of Forest Protection, College of Forestry, Sirsi – 581 401

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Summary of Project / Major Outcome /Achievements : For enhancing productivity of medicinal plants viz., Lemon grass, Citronella grass, Patchouli, Stevia, Tulsi, Coleus, Ashwagandha, Kalmegh and Aloe; seedling of selected medicinal plants (raised through various methods) were transplanted in the agro forestry system in Mango, Sapota and Cashew cropping system. Biological agents viz., Trichoderma viridis (TV), T. harzianum (TH), Psedomonas fluorescens (PF) and Arbuscular Mycorhizal Fungus (AMF) were also applied. Arbuscular Mycorhizal Fungus supported plant growth of Kalmegh, Coleus, Stevia and Aloe; Trichoderma viridiswas found suitable for Citrolena, Ashwagandha and Patchouli; Psedomonas fluorescens influenced Tulsi and Lemon grass. This influence was found significant and has good influence both on qualitative and quantitative characteristics resulting into better economic returns.

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Project No. : KR-20/2005-06

Year. : 2005-06

Project Title: Evaluation of intercropping potentially of MAPs under Pineapple (Annnas communis) based ecosystem in Uttara Kannada Dt. Of Karnataka

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Principal Investigator & Organization : Dr. A. Krishna, University of Agricultural Sciences, Dharwad, Krishinagar Campus, Dharwad – 580 005

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Summary of Project / Major Outcome /Achievements : Under Pineapple (Annnas communis) based ecosystem in Karnataka this study provides technical guidance to farmers who select appropriate medicinal and aromatic plants for intercropping in pineapple ecosystem. It was concluded that the sole crop recorded higher returns of 3.61 lakhs followed by Pacholi and lemon grass compared to other medicinal plants. Stevia given maximum returns (Rs. 27,800) followed by Lemon grass (Rs. 19220) and Vetivar grass (Rs. 18725). Least returns were obtained with Pineapple + Tulsi (Rs. 4845) and Aloe vera (Rs. 7656). The maximum total returns observed in the intercropping combination of Pineapple + Lemon grass and Pineapple + Citronella compared to others. The intercropping combination of Pineapple + Lemon grass recorded higher in benefit cost ration (3.64) followed by Cironella and Vetivar (3.44) compared to other medicinal plants. In general intercropping of aromatic grasses viz., Lemongrass, Citronella and Vetivar proved to be more suitable species to the pineapple based ecosystem.

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Project No. : KR-19/2005-06

Year. : 2005-06

Project Title: Novel approaches for management of wilt complex in Coleus forskohlii

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Principal Investigator & Organization : Dr. Yashoda R. Hegde, Department of Plant Pathology, College of Agriculture, Dharwad – 580 005

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Summary of Project / Major Outcome /Achievements : "For assessment of incidence of wilt complex in coleus, intensive survey was conducted in Coleus growing areas of Dharwad and Uttar Kannada districts of Karnataka. Disease was observed in all districts surveyed and highest wilt incidence was observed in nursery bed at Sirsi resulting in 80-100% mortality. Under field condition, 40% mortality was observed. Disease incidence was more in nursery compared to field. Pathogens associated with wilt complex were identified as Fusarium chlamydosporum Wollenw & Reink, Rhizochtonia bataticola (Tamb.) Butler and Sclerotium rolfsii Sacc. Among systemic fungicides, Carboxin @ 0.05% and 0.1% inhibited the mycelial growth completely. Among 18 plant extracts evaluated, Eupatorium odoratum L. showed maximum inhibition of mycelial growth (45.02%). The maximum reduction in colony growth of R. bataticola was observed in T. harzianum (Dharwad isolate) i.e. 79.63%. Field trails were conducted to develop the Integrated Disease Management (IDM) modules for management of wilt complex by combining cultural, biological, and chemicals. Pre-soaking of rooted cuttings in carboxin @ 0.1% followed by soil drenching at 30DAP were effective for management of wilt complex. The next best treatment was combined application of FYM @ 10t/ha + T. harzianum @ 25Kg/ha. "

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Project No. : KR-14/2005-06

Year. : 2005-06

Project Title: Conservation and propagation studies on indigenous medicinal plants of Anti-fertile properties

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Principal Investigator & Organization : Prof. S.K. Patil, Deptt. of Forest Utilization & Director of Instruction (Forestry), College of Forestry, Banavasi Road, Sirsi – 581 401 Uttar Kannada

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Summary of Project / Major Outcome /Achievements : For propagation studies on indigenous medicinal plants presowing seed treatment revealed significantly higher germination percentage over control. The maximum germination per cent (97.67 % and 95.33 % in Tarminatia bellrica and Terminalia arjuna, respectively) was in water treatment for 3 days. Mean daily germination differed significantly in various pre-sowing treatments. The maximum mean daily germination (3.69 and 3.72 in Terminalia bellirica and Terminalia arjuna, respectively) was observed in water treatment for 3 days. Similarly peak value, germination value and germination rate also differed significantly over control and highest in water treatment for 3 days. There was significant difference in growth and biomass parameters such as shoot length, root length, fresh and dry weight of Terminalia bellirica and Terminalia arjuna seedlings in water treatment for 3 days and this treatment showed increased seedling vigour Index by 133.09 and 160.44 per cent in Terminalia bellirica and Terminalia arjuna respectively.

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Project No. : KR-07/2005-06

Year. : 2005-06

Project Title: Ex-situ conservation and utilization of medicinal plants of Karnataka for sustainable development

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Principal Investigator & Organization : Dr. Ravishankar Rai V. Department of Studies in Microbiology, University of Mysore, manasagangothri, Mysore-570006

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Summary of Project / Major Outcome /Achievements : Nothapodytes foetida and Thalictrum dalzellii were conserved in field gene bank and also micro-propagated for mass propagation. The bioactive compounds camptothecin and berberin were isolated respectively from different plant parts as well as after micro-propagation and quantified through Thin Layer Chromatography (TLC) and High Performance Liquid Chromatography (HPTLC). In addition to this, medicinal plants viz., Memecylon malabaricum, Cochlospermum religiosum, Andrographis serpyllifolia, Rhus mysorensis, Ventilago maderaspatana, Boswellia serrate, Strychnos potatorum, Ancistrocladus heyneanus, Rotula aquatica were also evaluated for antibiotic activity, antioxidant activity, DPPH free radical scavenging ability and determination of the inhibitory activity towards lipid peroxidation. Methanolic extracts of roots and leaves were used for antibacterial activity and extracts for inhibition of beta-carotene bleaching.

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Project No. : KR-04/2005-06

Year. : 2005-06

Project Title: Studies on intercropping of medicinal and aromatic plants with horticultural crops

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Principal Investigator & Organization : Hemla Naik, AICRP (vegetables), University of Agricultural Sciences, Dharwad – 580 005

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Summary of Project / Major Outcome /Achievements : Intercropping of medicinal and aromatic plants with horticultural crops revealed that in general, medicinal and aromatic plants produce good vegetative growth under coconut plantation than open and mango plantations at Dharwad conditions (Northern Transitional zone of Karnataka). Patchouli, Lemongrass, Aloe vera, Tulsi, Japanese mint, Palmarosa, Citronella can be grown under coconut shade under Dharwad conditions. Stevia, Coleus, Ashwagandha, Khus and Kalmegh are best performers under open conditions. Higher active principle content can be obtained by cultivating Coleus, Stevia, Ashwagandha, Kalmegh and Khus in open conditions. Based on the present market prices, Stevia, Coleus, Khus and Patchouli can be recommended to farmers of Northern Transitional Zone in Karnataka state for large scale cultivation.

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